MAGUS CS3 Calibration Slide (0.01/0.05/0.1)

<p><strong>MAGUS CS3 calibration slide (0.01/0.05/0.1)</strong><br>

X-axis micrometer scales with 0.01/0.05/0.1mm scale division</p>

<p>Calibration slide for microscopes.<br />The slide has three horizontal micrometer scales of 100 divisions. The scale division is 0.01mm, 0.05mm, and 0.1mm, respectively.<br />The calibration slide (stage micrometer) is used to determine the magnification, the linear field of view of the microscope, and the division value of the eyepiece scales and reticles.<br />With a digital camera, the slide is used to calibrate image analysis software for each objective.</p>
<p><strong>Image analysis software calibration</strong></p>
<p>Calibration is performed for each objective that will be used in observations. The calibration slide is used to determine the calibration factor for the image analysis software.</p>
<p>Calibration procedure:</p>
<ol>
<li>Fix the calibration slide on the microscope stage.</li>
<li>Select the desired objective and adjust the maximum camera resolution.</li>
<li>Get a contrast image of the stage on the monitor screen and capture the image.</li>
<li>Select the “Calibration” mode in the image analysis software.</li>
<li>Click the mouse to define the maximum visible distance between the first and last division of the scale.</li>
<li>Enter the resulting scale size in real units into the image analysis software.</li>
<li>Enter the calibration name specifying the current objective magnification.</li>
<li>Check the result: in “Measurement” mode, measure a few divisions of the calibration slide scales in real units.</li>
<li>The image analysis software will save the calibration factor with the selected name. After that, it will automatically convert the results to the selected units of measurement.</li>
</ol>
<p><strong>Eyepiece scale calibration</strong></p>
<p>Eyepiece scale calibration is performed for each objective that will be used in observations. The calibration slide should be placed on the stage, and the microscope should be focused on the measuring scale. The eyepiece should be rotated in the eyepiece tube until the scales of the slide and eyepiece are aligned in parallel.</p>
<p>If you use low-magnification objectives, you should calculate how many divisions of the eyepiece built-in scale fit into the measuring scale of the slide.<br />If you use medium and high-magnification objectives, you should calculate how many divisions of the slide scale fit into the eyepiece scale.</p>
<p>The MAGUS CS3 calibration slide offers three micrometer scales with different division values. Calibration is most convenient when the slide scale spans the entire eyepiece scale. At low magnification, a scale with a division of 0.1mm or 0.05mm is used. At objectives with magnification higher than 20x, a measuring scale with 0.01mm divisions is used.</p>
<p>The calculation for each objective is performed according to the formula:<br />E=TL/A, where<br />E – division value of the eyepiece<br />T – division value of the stage specified on the stage micrometer<br />L – number of divisions of the stage micrometer<br />A – number of divisions of the eyepiece scale.</p>
<p>The resulting values from calculating the eyepiece scale division for each objective should be written down.</p>
<p>When measuring an object, you should first count the number of eyepiece scale divisions the object spans. The result is multiplied by the previously calculated scale value for the selected objective. The resulting value will be the true linear value of the object.</p>