MAGUS SPH2 Phase Contrast Device

MAGUS SPH2 Phase Contrast Device
For phase contrast microscopy

The MAGUS SPH2 Phase Contrast Device consists of a set of phase contrast slider compensators, a set of phase objectives, and a centering telescope.
The phase contrast device is used to study transparent, low-contrast samples that are not visible in the brightfield. Staining kills living cells. The main advantage of the technique is that it can be used to study unstained living organisms in their natural state. The technique is applied in medicine, ecology, pharmacology, agriculture, etc.

Phase contrast slider compensators
The phase contrast slider compensator is installed in the condenser slot. The slider compensator is a plate with two holes. One free hole for brightfield observations; the second hole has a phase insert that matches the objective in use.
The phase contrast device includes two slider compensators: one has an insert that works in tandem with 10x, 20x, and 40x objectives; the other insert works in tandem with a 100x objective. The mark on the insert matches the objective in use.
The slider compensator allows easy switching from brightfield observation to the phase contrast technique and vice versa.

Plan achromatic phase objectives
Phase objectives differ from regular objectives by having a phase ring in the plane of the exit pupil.
Plan achromatic phase objectives form an image that has the same focus at the center and at the edges, and therefore the distortion is negligible. Chromatic aberrations are corrected for blue and red, spherical aberration for green, and field curvature is corrected for 90% of the field of view diameter.
The 10x objective is designed to work with samples with or without cover slips. The 20x, 40x, and 100x objectives are used to observe samples with a 0.17mm cover slip. The 100x objective supports oil immersion. The objectives are designed for the tube length with infinity correction. The parfocal distance is 60mm.

Centering telescope
Using a centering telescope, the slider ring diaphragms are aligned with the phase rings of the objective.

Compatibility:
MAGUS Bio 260T Biological Microscope
MAGUS Bio DH260 Biological Digital Microscope

Package:
Phase contrast slider compensator for 10x, 20x, and 40x objectives with centerable rings
Phase contrast slider compensator for 100x objective with centerable rings
Infinite plan achromatic phase objective Plan 10x/0.25 Ph ∞/- WD 10.2mm, parfocal distance: 60mm
Infinite plan achromatic phase objective Plan 20x/0.40 Ph ∞/ 0.17 WD 4.8mm, parfocal distance: 60mm
Infinite plan achromatic phase objective Plan 40x/0.65 Ph ∞/ 0.17 WD 1.5mm, parfocal distance: 60mm
Infinite plan achromatic phase objective Plan 100x/1.25 oil Ph ∞/ 0.17 WD 0.22mm, parfocal distance: 60mm
Centering telescope