MAGUS Epi-FL06 Epifluorescence attachment, mercury lamp, B/G/UV/V/R/FITC/TRITC/DAPI fluorescence filter cubes

MAGUS Epi-FL06 Epifluorescence attachment, mercury lamp, B/G/UV/V/R/FITC/TRITC/DAPI fluorescence filter cubes
For fluorescence observations

The epifluorescence attachment is used to employ the fluorescence technique.
The fluorescence technique is based on the ability of substances to emit light when exposed to light of a certain wavelength. Some samples exhibit autofluorescence, while others must be treated with fluorochromes.

The attachment is installed in the optical path, between the microscope head and the stand. The attachment has a 6-position revolving nosepiece with fluorescence filters. Five fluorescence filter cubes can be installed on the nosepiece simultaneously (user-selectable). The required filter is introduced into the optical path by rotating the ring. One position of the nosepiece corresponds to brightfield observations. The side knobs are used to adjust the aperture of the field and aperture diaphragms. The set of fluorescence filter cubes includes filters for different fluorescence wavelengths: wideband blue (B), green (G), ultraviolet (UV), violet (V), narrowband red (R), and narrowband filters for DAPI, FITC, and TRITC fluorochromes.

The fluorescence excitation source is a mercury lamp. It has high brightness and a wide range of wavelengths. The lamp's spectrum is peak-like, which allows working with various fluorescent dyes. The mercury lamp is located in the lamphouse. The lamp housing dissipates heat, and the lamp does not overheat.

To use the fluorescence technique, use the MAGUS Epi-FL06 attachment in conjunction with the MAGUS PlanF S-Apo fluorescence objective set. The semi-apochromatic plan fluorescence objective improves the microscope's resolution and reduces chromatic and spherical aberrations. The image in the eyepieces and through the digital camera is sharper, has higher contrast, and is brighter than with a conventional achromatic plan objective.
The high numerical aperture of fluorescent objectives improves the microscope's resolution, but at the same time reduces the depth of field. Therefore, for observations with transmitted light, we recommend using regular objectives from the microscope set with a greater depth of field.

Compatibility:
MAGUS Bio 290T biological microscope

Package:
Epifluorescence attachment
B/G/UV/V/R/FITC/TRITC/DAPI fluorescence filter cubes
Mercury lamp with power supply
Protective screen for UV light

Specifications:
Excitation light source: mercury lamp
Fluorescence filters: filter type, excitation wavelength/dichroic mirror/emission wavelength: blue (B), 460–495 nm / 505 nm / 510 nm; green (G), 510–550 nm / 570 nm / 575 nm; ultraviolet (UV), 330–385 nm / 410 nm / 420 nm; violet (V), 400–410 nm / 455 nm / 460 nm; red (R), 620–650 nm / 660 nm / 670–750 nm
Fluorescence filters: fluorochrome, excitation wavelength/dichroic mirror/emission wavelength: DAPI, 360–390 nm / 415 nm / 435–485 nm; FITC, 460–495 nm / 505 nm / 510–550 nm; TRITC, 528–553 nm / 565 nm / 578–633 nm