MAGUS CSS2 Calibration slide for stereomicroscopes (XY0.05)

<p><strong>MAGUS CSS2 calibration slide for stereo microscopes (XY0.05)</strong><br>

X/Y axis micrometer scales with 0.05 mm scale division and a 10x10 mm reticle</p>

<p>A calibration slide for stereo microscopes.<br>
The slide has a 10x10 mm reticle engraved on it. In the center of the slide, there are reticles: two 200-division micrometer scales. The scale division is 0.05 mm, and the length of each scale is 10 mm. <br> 
The calibration slide (stage micrometer) is used to determine the magnification, the linear field of view of the microscope, and the division value of the eyepiece scales and reticles. <br> 
With a digital camera, the slide is used to calibrate the image analysis software for each objective.</p>

<p><strong>Calibration of image analysis software</strong></p>

<p>Calibration is performed for each objective that will be used in observations. The calibration slide is used to determine the calibration factor for the image analysis software. </p>

<p>Calibration procedure:</p>

<ol>

<li>Fix the calibration slide on the microscope stage.</li>
<li>Select the desired objective and set the camera to maximum resolution.</li>
<li>Get a contrast image of the stage on the monitor screen and capture the image.</li>
<li>Select the "Calibration" mode in the image analysis software.</li>
<li>Click the mouse to define the maximum visible distance between the first and last divisions of the scale.</li>
<li> Enter the resulting scale size in real units into the image analysis software.</li>
<li> Enter the calibration name by specifying the current objective magnification. </li>
<li>Check the result: in "Measurement" mode, measure a few divisions of the calibration slide scales in real units.</li>
<li> The image analysis software will save the calibration factor under the selected name. After that, it will automatically convert the results to the selected units of measure.</li>

</ol>

<p><strong>Calibration of the eyepiece scale</strong></p>

<p>Calibration of the eyepiece scale is performed for each objective that will be used in observations. The calibration slide should be placed on the stage, and the microscope should be focused on the measuring scale. The eyepiece should be rotated in the eyepiece tube until the slide and eyepiece scales are aligned in parallel.</p>

<p>The calculation for each objective is performed according to the formula: <br> 
Е=ТL/A, where<br>  
E – eyepiece division value<br>  
Т – stage division value specified on the stage micrometer (0.05 mm) <br> 
L – number of divisions of the stage micrometer <br> 
А – number of divisions of the eyepiece scale.</p>

<p>The resulting values of calculating the eyepiece scale division for each objective should be noted.</p>

<p>When measuring an object, you must first count the number of eyepiece scale divisions that the object spans. The result is multiplied by the previously calculated scale value for the selected objective. The resulting value will be the true linear value of the object.</p>